Study on preparation ，safety and anti-tumor activity of apigenin silk fibroin nanoparticles / 中国药房

OBJECTIVE To prepare apigenin silk fibroin（API@SF）nanoparticles and to evaluate their safety and anti-tumor activity. METHODS API@SF nanoparticles were prepared by nanoprecipitation method ，and their morphology ，particle size ，Zeta potential，drug loading amount and in vitro release were characterized. The safety of nanoparticles was evaluated by hemolysis test and HE staining. MTT assay was adopted to evaluate inhibitory effects of API@SF nanoparticles on breast cancer 4T1 cells in mice. RESULTS The prepared API@SF nanoparticles were spherical with uniform distribution. The average particle size was 406.61 nm， the polydispersity index was 0.154，the Zeta potential was －18.4 mV，and the average drug-loading amount was 5.20％. The in vitro release results showed that the release rate of the nanoparticles was relatively fast in the release medium of pH 5.0 and relatively slow in the release medium of pH 7.4. Results of hemolysis test and HE staining showed that the nanoparticles had good biocompatibility. Results of MTT assay showed that the inhibitory effect of API@SF nanoparticles on 4T1 cells was significantly higher than that of API raw materials （P＜0.05），and its mechanism may be related to increasing the level of reactive oxygen species in cells. CONCLUSIONS API@SF nanoparticles are prepared successfully ，which possess good safety and anti-tumor activity.

Clinical analysis of no tension repair in the treatment of 630 patients with inguinal hernia / 中国基层医药

Objective To observe the effect of no tension repair in the treatment of inguinal hemia.Methods Notension hernia repair was used to treat 630 patients with inguinal hernia(648 sides):primary indirect inguinal hernia in 604 cases( 18 cases of bilateral),primary direct inguinal hernia in 15 cases(2 cases complicated with indirect inguinal hernia,1 case of double direct inguinal hernia complicated with the right indirect hernia),recurrent hernias( 11cases).The mesh plug and patch of American Bard Company production was selected as hernia repair material for inguinal hernia repair.The operation time,the wound pain day,postoperative ambulation time,complications,and the recurrence rate was observed.Results 630 cases were all successful in operation,and the operation time was 40 ～110min,average operation time about 50min.6 -24h after operation all patients were got out of bed.29 cases of the postoperative urinary retention,indwelling catheter 24 hours to 1 week;6 cases of the fat liquefaction under the incision;3 cases of postoperative foreign body sensation;recurrence in 2 cases(3 sides),1 case of bilateral infected and recurrence ; 11 cases of local swelling incision; 16 cases of incision with mild foreign body sensation.8 days after operation all patients were discharged.Conclusion The no tension repair in the treatment of inguinal hernia had good effect and low recurrence.

Diagnostic value of the levels of white cell count and C-reactive protein for paediatric appendicitis / 中国基层医药

Objective To evaluate the accuracy of white cell count(WCC) and C-reactive protein(CRP) for diagnosing severity of acute paediatric appendicitis.Methods Clinicopathologic characters,WCC,CRP,histology were analyzed in 150 children undergoing open appendectomy for acute appendicitis.Results Appendix was grossly inflamed in 123 of which 24 had perforation.Histology revealed simple acute appendicitis in 99(66.0％),gungnenous appendicitis in 24(16.0％) and the rest were normal.The duration of symptoms(4.6±5.6) time,temperature (38.3±0.9)℃,WCC[(15.6±4.7) × 109/L]and CRP[(1610±653)mg/L]were significantly worse in the perforated group(P ＜ 0.01).WCC had a higher accuracy and sensitivity than CRP in diagnosing simple acute appendicitis.The combined sensitivities of WCC and CRP were 93％ and 99％ for diagnosing simple acute appendicitis and a perforated appendix,respectively.Conclusion Accuracy of WCC was higher than CRP for diagnosing simple acute appendicitis.The combined sensitivity of WCC and CRP increases for simple acute appendicitis as well as perforated appendix.

Postoperative intestinal obstruction:a clinical analysis of 89 cases / 中国基层医药

Objective To investigate the principles of diagnosis and treatment of postoperative intestinal obstruction.Methods The clinical datas of 89 cases of postoperative intestinal obstruction treated from 2001 to 2005 were analyzed retrospectively.Results All the 89 cases were cured without severe complications such as intestinal fistula and no death.The early postoperative inflammatory intestinal obstruction occurred in 2 cases.Recurrent intestinal obstruction occurred in 2 cases.Conclusions Intestinal obstruction can be caused by operation in abdomen.The diagnosis depends on the medical history,clinical manifestations,abdominal plain X-ray and computed tomography.Therapeutic procedure and operative opportunity play an important role in the treatment of postoperative intestinal obstruction.Non-operative approach should be the first choice for early postoperative inflammatory intestinal obstruction.Prompt surgery is clearly indicated and valuable for acute or being strangulative obstruction.Active operative treatment should be considered in recurrent chronic intestinal obstruction.